Table 1.
List of Primers, Probes, and Concentrations Used in HybProbe Assays
| Locus | Primer locations and probe sequences* | Primer (μmol/L) |
|---|---|---|
| HPA 1 | tttatgctccaatgtacggggtaaactcttagctattgggaagtggtagggcctgcaggaggtagagagtcgccatagctctgatt-gctggacttctctttgggctcctgtcttacaggccctgcctctgggctcacctcgctgtgacctgaaggagaatctgctgaaggat-aactgtgccccagaatc (189) | 0.2/0.2 |
| LC RED 640-cctgcctccgggctcac-P | ||
| ggacttctctttgggctcctgtcttacagg-FITC | ||
| HPA 2 | ggctgacctcgctgcctcttggtgccctgcgtggtcttggcgaactccaagagctctacctgaaaggcaatgagctgaagaccc-tgcccccagggctcctgacgcccacacccaagctggagaagctcagtctggctaacaacaacttgactgagctccccgctgg-gctcctgaatgggctggagaatctcgacacccttctcctcc (207) | 0.2/0.2 |
| tcctgacgcccacacccaa-FITC | ||
| LC RED 640-ggagaagctcagtctggctaacaacaacttgactga-P | ||
| HPA 3 | ctgggcctgaccactcctttgcccccccaggtggactgggggctgcccatccccagcccctcccccattcacccggcccatca-caagcgggatcgcagacagatcttcctgccagagcccgagcagccctcgaggcttcaggatccagttctcgtagtga (160) | 0.2/0.2 |
| tgcccatccccagcccc-FITC | ||
| LC RED 640-cccccattcacccggcccatcac-P | ||
| HPA 4 | aagaatttctccatccaagtgcggcaggtggaggattaccctgtggacatctactacttgatggacctgtcttactccatgaaggatg-atctgtggagcatccagaacctgggtaccaagctggccacccagatgcgaaagctcaccagtaacctgcggattggcttcggg-gcatttgtggacaagcctgtgtcaccatacatgtatatctccccacc (218) | 0.15/0.5 |
| cccagatgcgaaagctcacca-FITC | ||
| LC RED 640-taacctgcggattggcttcgggg-P | ||
| HPA 5 | atgagtgacctaaagaaagaggaaggaagagtctacctgtttactatcaaagaggtaaaaaaaaaaaaataaactaatagtttaatt-tgctttagtactggtaatttaacttgcatttggaaagaaaaatttattattattgaatgataatttgcacagatagtatggtttacatttc (178) | 0.05/1.0 |
| ctacctgtttactatcaaagaggtaa-FITC | ||
| LC RED 640-aaaaaaaataaactaatagtttaatttgctttagtactgg-P | ||
| HPA 6 | tgggatcccagtgtgagtgctcagaggaggactatcgcccttcccagcaggacgaatgcagcccccgggagggtcagcccgt-ctgcagccagcggggcgagtgcctctgtggtcaatgtgtctgccacagcagtgactttggcaagatcacgggcaagtactgcga-gtgtgacgacttct (180) | 0.15/0.5 |
| cagcccccgcgagggtcag-FITC | ||
| LC RED 640-gtctgcagccagcggggcgagtgcct-P | ||
| HPA 15 | attttggcttatttcaaaatgtatcagttcttggttttgtgatgtttatatttattatcttgacttcagttacaggatttaccaagaatttgaagtaac-tgtacctgattctatcacttcttgggtggctactggttttgtgatctctgaggacctgggtcttggactaacaactactccagt (183) | 0.2/0.2 |
| acttcagttccaggatttaccaa-FITC | ||
| LC RED 640-aatttgaagtaactgtacctgattctatcacttctt-P |
*
Primer locations are indicated by underlined sequences. Italicized sequences indicate that these particular primers had TCCT engineered onto the 5′ end of oligonucleotide covering these nucleotides. Number in parentheses indicates the length of the amplicon in base pairs. Listed after the amplicon sequences are the detection probe and then the anchor probe. Nucleotides in bold in the detection probe indicate the locations of the SNPs.