Table 3.
HT29 cells | Ct* | CV |
---|---|---|
10,000 | 13.0 ± 0.29 | 0.022 |
1,000 | 16.2 ± 0.56 | 0.035 |
100 | 19.5 ± 0.37 | 0.019 |
10 | 22.1 ± 0.74 | 0.034 |
Threshold cycles (Ct) are expressed as means± SD.
20N-PCR assays performed on the same cDNA in six triplicate experiments.