Table 1.
Ct Values of Case 2 in MethyLight for CDKN2A (p16)
| Different MethyLight runs
|
Mean of Ct values | SD(M) | ||||||
|---|---|---|---|---|---|---|---|---|
| M1 | M2 | M3 | M4 | M5 | ||||
| Different bisulfite treatments | B1 | 31.35 | 31.15 | 31.02 | 31.17 | 31.20 | 31.18 | 0.12 |
| B2 | 30.86 | 30.79 | 30.49 | 30.82 | 30.75 | 30.74 | 0.15 | |
| B3 | 31.41 | 31.19 | 31.42 | 31.33 | 31.22 | 31.31 | 0.11 | |
| B4 | 31.05 | 31.04 | 30.95 | 31.07 | 30.67 | 30.96 | 0.17 | |
| B5 | 31.07 | 30.94 | 31.07 | 31.15 | 31.18 | 31.08 | 0.09 | |
| B6 | 30.46 | 30.49 | 30.54 | 30.75 | 30.53 | 30.55 | 0.11 | |
| B7 | 30.41 | 30.22 | 30.09 | 30.18 | 30.33 | 30.25 | 0.13 | |
| Mean of Ct values | 30.94 | 30.83 | 30.80 | 30.92 | 30.84 | |||
| SD(B) | 0.39 | 0.36 | 0.45 | 0.39 | 0.36 | |||
Each of B1 through B7 represents each bisulfite-converted sample, and each of M1 through M5 represents each of MethyLight assay results on 5 different days. SD(M) refers to MethyLight-to-MethyLight SD within each sample, and SD(B) refers to bisulfite-to-bisulfite SD within each MethyLight run.