Table 2.

Standard Deviations (SD) of Ct Values in MethyLight

		CDKN2A		MLH1		MGMT		ACTB		COL2A1
		Mean of SD		Range		Mean of SD		Range		Mean of SD		Range	Mean of SD*	Range	Mean of SD*	Range
Case 1	SD(B)			0.41	0.21–0.67			0.39	0.24–0.82	0.26	0.20–0.37
	SD(M)			0.96	0.58–1.1			0.45	0.14–0.93	0.19	0.095–0.44
Case 2	SD(B)	0.39	0.36–0.45			0.48	0.31–0.75	0.40	0.20–0.54	0.33	0.19–0.46
	SD(M)	0.12	0.093–0.17			0.56	0.37–0.86	0.61	0.44–0.78	0.27	0.15–0.48
Case 3	SD(B)	0.51	0.40–0.65			0.21	0.10–0.31	0.53	0.39–0.76	0.19	0.14–0.32
	SD(M)	0.51	0.36–0.67			0.17	0.076–0.29	0.50	0.27–0.74	0.19	0.13–0.21
Case 4	SD(B)	0.35	0.34–0.36	0.38	0.23–0.62			0.33	0.14–0.55	0.18	0.15–0.22
	SD(M)	0.17	0.089–0.31	0.48	0.23–0.97			0.46	0.28–0.67	0.16	0.052–0.26

^*Paired t-test, P = 0.0004. 

Blanks indicate negativity for methylation in a particular case-marker combination. 

Abbreviations: SD(B), bisulfite-to-bisulfite SD within each run; SD(M), run-to-run SD within each bisulfite-treated sample.