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Effect of different fixatives on mRNA recovery of the housekeeping gene EF-1a. Cryo-sections of rabbit appendix were incubated with a Cy3-conjugated anti-vimentin antibody for 5 minutes (one-step direct method). Each bar represents the mean Ct value and the SD of six identically treated samples. Although the different fixatives influenced the mRNA preservation only marginally, ethanol gave significantly better mRNA yields than Carnoy’s medium (P < 0.05).
