Skip to main content
. 2006 May;8(2):246–253. doi: 10.2353/jmoldx.2006.050096

Figure 3.

Figure 3

Effect of the incubation period on EF-1a mRNA recovery. Cryo-sections of rabbit appendix were fixed in ethanol for 2 minutes and incubated with antibody solution for different periods. Prolongation of the incubation period from 5 to 60 minutes leads to a drastic loss of detectable mRNA copies, corresponding to significantly increased Ct values in real-time PCR (P < 0.05; asterisks). The difference between the 5- and 15-minute samples is 9.0 Ct units, indicating that within only 10 minutes of additional incubation, there is a loss of 99.8% of specific mRNA. Bars represent means and SD of six samples per group.