Figure 5.

Influence of different staining and labeling techniques on ratios between the target gene vimentin and the housekeeping gene EF-1a. Although samples that underwent labeling or staining techniques that require just 5 minutes of incubation show ratios almost identical to those obtained from untreated tissues, we found a significant shift of ratios in tissue sections that were incubated for 15, 30, or 60 minutes compared with untreated tissue (P < 0.05; asterisks). Each bar represents ratios and SD of six identically treated samples.