Table 2.
Threshold Cycles of the Control Genes and BCR-ABL in CML Samples (n = 12)
| Genes | Ct (mean ± 2SD)* | ΔCt (BCR-ABL − control) |
|---|---|---|
| BCR-ABL | 26.58 ± 3.25 | — |
| ABL1 | 31.96 ± 2.75 | −5.38 |
| ABL2 | 28.94 ± 3.07 | −2.36 |
| ACTB | 20.02 ± 2.43 | +6.56 |
| B2M | 19.39 ± 2.65 | +7.19 |
| G6PD | 26.07 ± 3.09 | +0.51 |
| GAPDH | 22.12 ± 2.30 | +4.46 |
| GUSB | 26.32 ± 1.71 | +0.26 |
| HPRT | 24.66 ± 1.62 | +1.92 |
| PGK | 24.14 ± 2.05 | +2.44 |
| TBP | 28.57 ± 3.11 | −1.99 |
*
The Ct values for individual specimen are calculated based on six replicate reactions from two experiments. Data shown are means ± 2SD for 12 CML samples.