Table 4.

Reasons for Exclusion

Genes	Main reasons for exclusion
ABL1	Much higher Ct than BCR-ABL, different levels in CML and non-CML cells, different degradation kinetics from BCR-ABL, and being a translocation partner of BCR-ABL
ABL2	Different levels in CML and non-CML cells, and ability to detect the ABL portion of BCR-ABL
ACTB	More abundant than BCR-ABL and unusual amplification curve
B2M	More abundant than BCR-ABL and different levels in CML and non-CML cells
GAPDH	More abundant than BCR-ABL and different levels in CML and non-CML cells
G6PD	Presence of molecular variants and X chromosome location
HPRT	Different levels in CML and non-CML cells
PGK	Different levels in CML and non-CML cells
TBP	Different levels in CML and non-CML cells