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. 2006 May;8(2):282–287. doi: 10.2353/jmoldx.2006.050026

Figure 1.

Figure 1

Design of single tube real-time TaqMan assay for the detection of two common ML IV mutations. Vertical green bars represent 14 MCOLN gene exons. A duplex PCR reaction amplifies a fragment from exon 3 or the junction fragment created by the 511del6434 mutation. Three probes are included per reaction. The VIC probe (orange) detects the WT IVS3 allele and absence of the 511del6434. The FAM probe (dark green) detects the presence of the mut IVS3-2A>G allele, whereas the TET probe (red) detects the 511del6434 allele when the PCR product using primers that flank the deletion is amplified.