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. 2006 Jul;8(3):342–350. doi: 10.2353/jmoldx.2006.050133

Figure 2.

Figure 2

Luciferase assays with LTBP-1L promoter reporter constructs having the four haplotypes, using ovarian cancer RMUG-S cells. A: Schematic representation of luciferase reporter constructs containing a −2211 to +54 promoter region with different haplotypes at the SNP sites. B: The relative luciferase activities of reporter plasmids with the different haplotypes. Each column represents the mean of three independent experiments, each done in triplicate; bars, ±SD (*P < 0.01 compared with the C-C construct). C: Luciferase assays to examine the role of Sp1 in LTBP-1L promoter. Luciferase plasmids with G-A or C-C haplotype were co-transfected with blank vector, Sp1, or dominant-negative Sp3 expression vector to inhibit Sp1 activity. The relative luciferase activities in each reporter plasmid are shown, based on the luciferase activity in C-C reporter plasmid transfected with blank vector, normalized to 1. Each column represents the mean of three independent experiments, each done in triplicate; bars, ±SD (*P < 0.0001 compared with the G-A blank, #P < 0.0001 compared with the C-C blank).