Figure 3.

EMSAs for Sp1 binding to the LTBP-1L promoter. The nucleotide sequences of the probes with SNP at −202 are shown. Underlining indicates the Sp1 binding motif. The asterisk shows the SNP site. Recombinant Sp1 proteins were incubated with ³²P-labeled probes with G or C at −202, respectively, and separated by electrophoresis. Antibodies and competitors used are shown. Homo, homologous competitors of −202G probe. Arrow B represents specific binding complexes of Sp1 and arrow S exhibits the supershifted bands. Arrow F shows free probes.