Table 3.
B-Cell Clonality by PCR for IGH by Diagnosis
| Diagnosis | Number of cases studied | Number of positive cases (%) | Number of positive cases detected by specific primer sets (%)
|
||||
|---|---|---|---|---|---|---|---|
| FR1 | FR2 | FR3 | DH1-6 | DH7 | |||
| AILT | |||||||
| Without B-cell proliferation† | 44 | 13 (30) | 3 (7) | 2 (5) | 6 (14) | 4 (9) | 6 (14) |
| With B-cell proliferation | 12 | 6 (50) | 5 (42) | 3 (25) | 4 (33) | 1 (8) | 2 (17) |
| Total | 56* | 19 (34) | 8 (14) | 5 (9) | 10 (18) | 5 (9) | 8 (14) |
| PTCL-NOS | |||||||
| Without B-cell proliferation‡ | 46 | 15 (33) | 8 (17) | 3 (7) | 8 (17) | 10 (22) | 4 (9) |
| With B-cell proliferation | 11 | 5 (45) | 2 (18) | 1 (9) | 3 (27) | 2 (18) | 3 (27) |
| Total | 57* | 20 (35) | 10 (18) | 4 (7) | 11 (19) | 12 (21) | 7 (12) |
Framework region (FR) primer sets amplified rearrangements in IGH between variable segment (VH), diversity segment (DH), and joining segment (JH). DH primer sets amplified rearrangements between DH and JH.
*
Two cases of AILT and two cases of PTCL-NOS were not studied because of insufficient DNA.
†
One case yielded oligoclonal products with FR1 only and was scored as negative.
‡
One case yielded clonal products with FR1 and oligoclonal products with DH1-6 and was scored as positive.