Detection of the K103N mutation by the ViroSeq system in samples with different levels of K103N-containing HIV-1 variants. Samples with subtype A, C, and D were analyzed with the LigAmp assay to determine the percentage of HIV-1 variants in the viral population that had the K103N mutation. Samples with >1% K103N detected in the LigAmp assay were divided into categories for each subtype with >20% K103N, >10% but ≤20% K103N, >5% but ≤10% K103N, and >1% but ≤5% K103N. The graph indicates the percentage of samples in each category that had K103N detected using the ViroSeq system. The number of samples tested in each sample subset is indicated below each bar.