Table 3.

Assay Linearity and Effect of Analysis of Melanoma Cell (SK-MEL-28) Titrations with and without Lymphoid RNA Matrix

	Dilutions without lymphoid matrix		Dilutions with lymphoid matrix
	Slope (regression line)	Y-intercept (95% CI)	Slope (regression line)	Y-intercept (95% CI)
MART-1	1.01 ± 0.07	−145–170	1.02 ± 0.14	−295–342
Tyrosinase	0.98 ± 0.05	−330–690	0.45 ± 0.02	−97–257

Regression lines for repeated measurements of four different concentrations of RNA from SK-MEL-28 melanoma cells (each in five replicates) diluted in water (without lymphoid matrix) and in lymph node RNA (with lymphoid matrix). See also description in Material and Methods.