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. 1998 Feb 3;95(3):1073–1078. doi: 10.1073/pnas.95.3.1073

Figure 1.

Figure 1

Rat liver cytosol contains two polypeptides, P200/myosin II and P190, that are recognized in immunoblots by the AD7 mAb: A cytosolic protein fraction (LCP) devoid of P200 efficiently supports the release of post-Golgi vesicles. (A) Equal aliquots (200 μg of protein) of total cytosol (lanes a and d), LCP (lanes b and e), and an immunoprecipitate from the total cytosol obtained with an anti-myosin II antibody (lane c) were analyzed by gel electrophoresis followed by Western blot with the AD7 anti-P200 (lanes a–c) or M3A5 anti-βCOP (lanes d and e) mAbs. (B) Aliquots of a Golgi fraction were incubated for 1 hr in standard reaction mixtures containing ATP (1 mM) with either total cytosol (○—○) or LCP (•—•) at the protein concentrations indicated. VSV-G protein release is expressed as percentage of the VSV-G radioactivity initially associated with the Golgi membranes (4).