Table 2.
Putative serine proteases and serpins
| Cluster code | Nucleotide | EST | E value | Homology | Putative function |
|---|---|---|---|---|---|
| I.1 | 319 | 4A3B-aad-d-12-F | 1e-19 | Serine protease 14D (A.g.) | Activation of PPO cascade |
| 206 | 4A3B-aad-d-12-R | 1e-05 | Easter (D.m.) | ||
| 245 | 4A3B-aax-c-11-F | 2e-06 | Serine protease 14D (A.g.) | ||
| 304 | 4A3B-aax-c-11-R | 3e-06 | Serine protease 14D (A.g.) | ||
| 366 | 4A3A-aaq-c-04-F | 3e-20 | Serine protease 14D (A.g.) | ||
| 356 | 4A3A-aaq-c-04-R | 8e-12 | Serine protease 14D (A.g.) | ||
| 288 | 4A3A-aat-a-03-F | 5e-19 | Serine protease 14D (A.g.) | ||
| 4A3A-aat-a-03-R | 3e-12 | Serine protease 14D (A.g.) | |||
| I.2 | 342 | 4A3A-aal-a-11-F | 5e-17 | Coagulation factor D (T.t.) | Coagulation cascade |
| 338 | 4A3A-aal-a-11-R | 6e-05 | Trypsin like serine protease (C.f.) | ||
| 379 | 4A3A-P2G4-R | 5e-12 | Masquerade like protein (P.l.) | ||
| I.3 | 340 | 4A3A-aam-e-02-F | 2e-05 | Easter (D.m.) | Developmental |
| 241 | 4A3A-aam-e-02-R | 4e-07 | Factor C (C.r.) | Coagulation cascade | |
| I.4 | 181 | 4A3B-aaa-a-02-F | 3.0 | Trypsin-like (C.f.) | Complement |
| 277 | 4A3B-aaa-a-02-R | 4e-10 | Trypsin-like (C.f.) | ||
| I.5 | 301 | 4A3A-aal-c-02-R | 2e-05 | Hemocyte protease-1 (M.s.) | Serine protease |
| I.6 | 391 | 4A3A-aaq-f-04-R | 0.003 | Serine protease 14D (A.g.) | Serine protease |
| 447 | 4A3A-aaq-f-04-R | 6e-15 | Elastase precursor (P.o.) | ||
| I.7 | 238 | 4A3B-aad-b-06-F | 4e-15 | Serine protease 14D (A.g.) | Activation of PPO cascade |
| I.8 | 285 | 4A3B-aau-g-07-R | Identical | Serine protease 22D (A.g.) | Adhesive serine protease |
| I.9 | 431 | 4A3B-aau-c-06-F | 1e-04 | Serpin (P.l.) | Coagulation factor inhibitor |
| 274 | 4A3B-aau-c-06-R | 8e-10 | Serpin (P.l.) | ||
| 356 | 4A3B-aag-b-09-R | 4e-05 | Serpin (P.l.) | ||
| 637 | 4A3A-P4E1-F | 2e-09 | Serpin (P.l.) | ||
| 411 | 4A3A-P4E1-R | 0.002 | Serpin (R.n.) | ||
| I.10 | 513 | 4A3A-aap-b-5-F | Identical | Ag.serpin (A.g.) | Serine protease inhibitor |
| 412 | 4A3A-aap-b-5-R | Identical | Ag.serpin (A.g.) | ||
| 557 | 4A3A-aas-g-03-F | Identical | Ag.serpin (A.g.) | ||
| 416 | 4A3A-aas-g-03-R | Identical | Ag.serpin (A.g.) | ||
| I.11 | 594 | 4A3A-P6H1-F | 1e-12 | Serpin (H.c.) | Serine protease inhibitor |
| 448 | 4A3A-P6H1-R | 1e-05 | Serpin (A.t.) | ||
| I.12 | 430 | 4A3A-aap-b-10-F | 4e-08 | Antiplasmin serpin (B.t.) | Serine protease inhibitor |
Clusters with similarity to putative immunity-related genes (note that the following legend applies to Tables 3 and 4 as well). The clusters were assigned short numerical codes and are displayed in the same order as presented in the main text. Their EST identifiers indicate the cell line origin (4A-3A or 4A-3B), the position in the plates of arrayed cDNA libraries, and finally the vector primer used for sequencing [reverse (R) and forward (F)]. In the great majority of clones, the R primer is near the 5′ end of the ESTs, and the F primer is near the 3′ end. The blastx hits with the lowest E values (implying the most significant similarities) are indicated in the table; inference of putative function is derived from these and additional protein matches with highly significant similarities. Previously known A. gambiae genes are marked “identical.” The organisms corresponding to listed similar proteins are indicated with italics initials in the homology column: A.g., A. gambiae; D.m., Drosophila melanogaster; T.t., Tachypleus tridentiatus; C.f., Ctenocephalides felis; C.r., Carcinoscorpius rotundicauda; M.s., Manduca sexta; P.o., Paralichthys olivaceus; P.l., Pacifastacus leniusculus; R.n., Rattus norvegicus; H.c., Hyalophora cecropia; A.t., Arabidopsis thaliana; B.t., Bos taurus.