. 2000 May 23;97(12):6625–6629. doi: 10.1073/pnas.120168697

Table 3.

Increase in Mks1p expression increases [URE3] incidence

Plasmids			USA⁺ per 10⁶ cells
CEN P_MKS1	+	2 μ P_ADH1	2
CEN P_MKS1	+	2 μ P_ADH1URE2	30
CEN P_MKS1	+	2 μ P_ADH1URE2ΔApaI	1,200
CEN P_MKS1MKS1	+	2 μ P_ADH1	11
CEN P_MKS1MKS1	+	2 μ P_ADH1URE2	1,500
CEN P_MKS1MKS1	+	2 μ P_ADH1URE2ΔApaI	32,000

Single-copy plasmids expressing Mks1p from its native promoter were used to obtain a modest increase in expression of this protein. Transformants of YHE869 (MATα ura2 leu2Δ∷hisG trp1Δ∷hisG [ure-o]) with the indicated plasmids were grown without tryptophan or leucine and suspended in water, and dilutions were plated on SD + USA. Number of colonies formed after 5 days is shown. Plasmids: pH7 = 2 μ LEU2 P_ADH1, pH14 = 2 μ LEU2 P_ADH1URE2, pH438 = 2 μ LEU2 P_ADH1URE2ΔApaI, pH417 = CEN TRP1 P_MKS1, pH431 = CEN TRP1 P_MKS1MKS1. The experiment was carried out four times with similar results; typical data are shown.