Table 2.
Characterization of P. aeruginosa isolates from intubated patients
Isolates | Patients | Genotype | Site of Isolation | Productionc of | Elastasea(OD495/OD660) | Productiona of | Adhesiona (%) | Biofilma (%) | ||
Proteases | Rhamnolipids | 3-oxo-C12-HSL (μM) | C4-HSL (μM) | |||||||
61G3d | 8 | D | TA | + + + | BDb | 0.5 | 0.51 | 15 | 88 | 31 |
61E8 | 8 | D | TA | + + + | BD | 0.59 | 0.41 | 24 | 88 | 11 |
60A3d | 8 | B | TA | + + + | + | 0.68 | 0.33 | 7.8 | 100 | 13 |
60B5 | 8 | D | TA | + + + | BD | 0.65 | 0.36 | 5 | 139 | 33 |
63E8 | 8 | B | ID | + + + | + | 0.3 | 1.3 | 2.4 | 116 | 83 |
63E9 | 8 | B | ID | + + + | + + | 0.34 | 2.5 | 1.1 | 111 | 105 |
63E11d | 8 | B | ID | + + + | + + + | 0.29 | 0.19 | 2.9 | 105 | 89 |
63G3 | 8 | B | ID | + + + | + | 0.24 | 0.63 | 15 | 115 | 59 |
63G6 | 8 | D | ID | + + + | BD | 0.3 | 0.36 | 3 | 120 | 98 |
63G8 | 8 | B | ID | + + + | + + | 0.2 | 0.21 | 3.3 | 106 | 125 |
63H2 | 8 | B | ID | + + + | + + + | 0.12 | 2.7 | 2.9 | 137 | 74 |
62F2 | 8 | D | ID | + + + | BD | 0.32 | 0.37 | 3.1 | 99 | 10 |
62F3d | 8 | D | ID | + + + | + | 0.22 | 0.17 | 2.4 | 101 | 20 |
62G3 | 8 | D | ID | + + + | BD | 0.3 | 0.29 | 3.3 | 110 | 25 |
54A7 | 12 | C | TA | + + + | + + | 0.5 | 0.36 | 30 | 104 | 59 |
54A8 | 12 | B | TA | + + + | + | 0.63 | 0.41 | 19 | 115 | 49 |
54A9 | 12 | C | TA | + + + | + + + | 0.5 | 0.38 | 12 | 105 | 49 |
54B1 | 12 | C | TA | + + + | + | 0.64 | 0.77 | 30 | 99 | 60 |
54D5d | 12 | B | TA | + + + | + | 0.48 | 0.35 | 14 | 116 | 50 |
54D6d | 12 | B | TA | + + + | + + + | 0.43 | 0.31 | 18 | 80 | 53 |
54E1 | 12 | B | TA | + + + | + + | 0.72 | 1 | 7.9 | 121 | 58 |
55A1d | 12 | B | ID | + + | BD | 0.19 | 2.6 | 2.1 | 116 | 78 |
55A2 | 12 | B | ID | + + | + | 0.27 | 1.2 | 1.3 | 122 | 75 |
55A5 | 12 | B | ID | + + | + + | 0.26 | 2.5 | 2.8 | 137 | 102 |
55D2 | 12 | B | ID | + + | + | 0.27 | 2 | 3.4 | 118 | 68 |
55E4 | 12 | B | ID | + + | + + | 0.31 | 2.5 | 2.7 | 115 | 88 |
55F3d | 12 | B | ID | + + | + + + | 0.22 | 2.5 | 2.2 | 101 | 59 |
55G2 | 12 | B | ID | + + | + + | 0.17 | 4 | 2 | 117 | 84 |
55G4 | 12 | B | ID | + + | + | 0.19 | 1.3 | 1.6 | 112 | 103 |
55A6 | 12 | B | ID | + + | + + + | 0.25 | 2.1 | 3.4 | 126 | 109 |
56A1 | 13 | B | TA | + + + | + + | 0.64 | 0.44 | 6.4 | 85 | 46 |
59B3 | 13 | C | TA | + + + | + + | 0.45 | 0.3 | 7.9 | 88 | 39 |
59B4d | 13 | B | TA | + + + | + + | 0.47 | 0.16 | 9.5 | 83 | 48 |
59A1d | 13 | B | TA | + + + | + + + | 0.52 | 0.13 | 9.3 | 99 | 38 |
59B6 | 13 | P | TA | + + + | + + | 0.45 | 0.28 | 13.5 | 83 | 41 |
59C1 | 13 | P | TA | + + + | + + + | 0.52 | 0.27 | 11.3 | 106 | 77 |
59C2 | 13 | P | TA | + + + | + | 0.47 | 0.33 | 13.3 | 101 | 59 |
63A1 | 13 | B | ID | + + + | + + | 0.23 | 1.4 | 1.8 | 114 | 91 |
63A3d | 13 | B | ID | + + + | + + + | 0.3 | 0.33 | 3.1 | 137 | 77 |
61A12d | 13 | B | ID | + + + | + + + | 0.15 | 2.6 | 2.2 | 129 | 90 |
61G12 | 13 | B | ID | + + + | + + | 0.11 | 2.7 | 2.4 | 121 | 87 |
62C2 | 13 | B | ID | + + + | + + | 0.15 | 2.6 | 3.1 | 121 | 97 |
62C3 | 13 | B | ID | + + + | + + + | 0.08 | 1.3 | 3.3 | 143 | 86 |
a Values are mean of three independent experiments with a standard deviation of < 10%.
b BD, below detection.
c +++, high level; ++, intermediate level; +, low level.
d lasR and rhlR genes sequenced