Figure 5.

IFN activated DNA binding of transcription factors. Cells were treated with indicated IFNs (150 units/ml) for 30 min and EMSA was performed with ³²P-labeled ISRE (A and B) or pIRE (C). In A and B cytoplasmic and nuclear extracts (4 μg) from the same cells were used. None, no extract. “−” and “+” signs are similar to Fig. 2. Positions of specific complexes were indicated. In C nuclear extracts (3 μg) were used for EMSA.