Table 1.
Absolute Cell Counts in CBA/J and C57BL/6 Mice (Mean ± SEM)
| Control | Stress | SP | Control NK1-RA | Stress NK1-RA | |
|---|---|---|---|---|---|
| CBA/J mice | |||||
| TUNEL+ cells | |||||
| Bulge* | 3.4 ± 1.1 | 7.9 ± 1.7↑ | 6.9 ± 1.1↑ | 3.4 ± 1.1 | 2.8 ± 0.9 ↓ |
| Bulb† | 9.7 ± 2.6 | 13.7 ± 4.7 | 6.9 ± 2.9 | 2.6 ± 0.7 | 5.0 ± 1.7 |
| MHC II+‡ | 2.0 ± 0.3 | 7.0 ± 1.1 ↑ | 8.3 ± 1.2 ↑ | 3.3 ± 0.3 | 2.7 ± 0.3 ↓ |
| Mast cells | |||||
| Dermis total/ | 7.6 ± 0.5/ | 8.6 ± 0.7/ | 7.8 ± 0.4/ | ND | ND |
| Degranulated§ | 2.2 ± 0.3 | 4.1 ± 0.3 ↑ | 3.3 ± 0.3 ↑ | ||
| Subcutis total/ | 3.6 ± 0.4/ | 4.5 ± 0.5/ | 3.7 ± 0.4/ | ND | ND |
| Degranulated§ | 1.0 ± 0.2 | 1.9 ± 0.2 ↑ | 1.3 ± 0.2 | ||
| C57BL/6 mice | |||||
| TUNEL+ cells | |||||
| Bulge* | 6.7 ± 1.8 | 15.2 ± 1.7 ↑ | 21.7 ± 3 ↑ | 2.1 ± 0.4 | 7.5 ± 2.1 ↓ |
| Bulb† | 6.2 ± 1.1 | 14.8 ± 5.0 ↑ | 22.0 ± 3 ↑ | 6.4 ± 1.1 | 8.2 ± 1.3 ↓ |
| MHC II+‡ | 4.7 ± 0.6 | 11.8 ± 0.8 ↑ | 8.5 ± 0.9 ↑ | 7.0 ± 1.8 | 5.3 ± 0.5 ↓ |
| Mast cells | |||||
| Dermis total/ | 6.8 ± 0.4/ | 6.7 ± 0.9/ | 5.7 ± 0.4/ | ND | ND |
| Degranulated§ | 0.7 ± 0.1 | 4.1 ± 0.9 ↑ | 2.5 ± 0.3 ↑ | ||
| Subcutis total/ | 2.9 ± 0.3/ | 3.0 ± 0.2/ | 2.5 ± 0.3/ | ND | ND |
| Degranulated§ | 0.3 ± 0.1 | 1.8 ± 0.2 ↑ | 1.8 ± 0.2 ↑ |
*Bulge area of anagen VI/catagen I follicles covers 40-μm2 tissue.
†Bulb area of anagen VI/catagen I follicles covers 60-μm2 tissue.
‡Number of cell clusters per 12.5-mm epidermal length.
§Classified as degranulated when eight or more granules were seen outside the cell membrane.
ND, Not determined.
↑, Significant increase compared to nonstressed control (P ≤ 0.05).
↓, Significant decrease compared to stressed, non-NK1 RA-treated group (P ≤ 0.05).