Abstract
An experimental procedure developed to concentrate fungal viruses from large volumes of homogenized mycelia with water-soluble polymers, such as polyethylene glycol, possesses advantages over more conventional methods. Concentration of the Penicillium stoloniferum fast-moving virus from mycelial homogenates after addition of polyethylene glycol was rapid and produced large quantities of pure virus.
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Selected References
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- Banks G. T., Buck K. W., Chain E. B., Darbyshire J. E., Himmelweit F. Penicillium cyaneo-fulvum virus and interferon stimulation. Nature. 1969 Jul 12;223(5202):155–158. doi: 10.1038/223155a0. [DOI] [PubMed] [Google Scholar]
- Bozarth R. F., Wood H. A., Mandelbrot A. The Penicillium stoloniferum virus complex: two similar double-stranded RNA virus-like particles in a single cell. Virology. 1971 Aug;45(2):516–523. doi: 10.1016/0042-6822(71)90352-7. [DOI] [PubMed] [Google Scholar]
- CHARNEY J., MACHLOWITZ R., TYTELL A. A., SAGIN J. F., SPICER D. S. The concentration and purification of poliomyelitis virus by the use of nucleic acid precipitation. Virology. 1961 Nov;15:269–280. doi: 10.1016/0042-6822(61)90358-0. [DOI] [PubMed] [Google Scholar]
- Kanarek A. D., Tribe G. W. Concentration of certain myxoviruses with polyethylene glycol. Nature. 1967 May 27;214(5091):927–928. doi: 10.1038/214927a0. [DOI] [PubMed] [Google Scholar]
- Leberman R. The isolation of plant viruses by means of "simple" coacervates. Virology. 1966 Nov;30(3):341–347. doi: 10.1016/0042-6822(66)90112-7. [DOI] [PubMed] [Google Scholar]
- Loening U. E., Ingle J. Diversity of RNA components in green plant tissues. Nature. 1967 Jul 22;215(5099):363–367. doi: 10.1038/215363a0. [DOI] [PubMed] [Google Scholar]
- McSharry J., Benzinger R. Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation". Virology. 1970 Mar;40(3):745–746. doi: 10.1016/0042-6822(70)90219-9. [DOI] [PubMed] [Google Scholar]
- Moffitt E. M., Lister R. M. Detection of mycoviruses using antiserum specific for dsRNA. Virology. 1973 Mar;52(1):301–304. doi: 10.1016/0042-6822(73)90421-2. [DOI] [PubMed] [Google Scholar]
- Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]