Figure 4.

Validation of increased expression of Amd1 in mouse endometrial cancer. Quantitative, real-time PCR verifying overexpression of Amd1 in the five cancer RNA samples hybridized to GeneChips (Ca pools 1 and 2, and Ca A, Ca B, and Ca C), and validating increased expression of Amd1 in additional cancers (Ca D and Ca F). The average fold increase in Amd1 levels in specimens Ca A to Ca F (electronic pool represented by the average column) is within the range of fold increases seen for Ca pools 1 and 2 (biological pools). The fold increases were obtained by comparing Amd1 levels in each cancer to transcript levels in uninvolved normal endometrium from the same animal (see Materials and Methods).