Table 1.
PCR Primers Used for the Generation of Recombinant Proteins Issued from the Alternatively Spliced Domain of Human and Mouse Tn-C
| Recombinant protein | Primers (5′-3′) |
|---|---|
| hFNIII A4-D | F:CTGGGATCCCAGGTGCAGGAGGTCAACAAAGTG |
| R:CCCAAGCTTGGGGGCAAGTAGGGTTATTTCCAG | |
| hFNIII A4-C | R:TACAAGCTTGTAACAATCTCAGCCCTCAAG |
| hFNIII A4-B | R:GACAAGCTTGTCGTGGCTGTGGCACTGATG |
| hFNIII A4 | R:CACAAGCTTGTGGAGGCCTCAGCAGAGTACTG |
| mFNIII FL | F:AAGGAATTCCTTCTGAGGTGTCCCCTCCCAAA |
| R:AACTCGAGGGAATGGGTACAGGAGTCCAAT | |
| mFNIII SO | F(1):AAGGAATTCCTTCTGAGGTGTCCCCTCCCAAA |
| R(5):AACCTCGAGGCCTTCACACGTGCAGGCTTGC | |
| F(6):ACGAATTCTGTCGACGGCCATGGGTTCTCCGAAGG | |
| R(8):AACTCGAGGGAATGGGTACAGGAGTCCAAT | |
| mEGF | F:AACGAATTCGCTGTGTCTGTGAACCAGGCTGG |
| R:TCCTCGAGAGCAGTCTATCCCTGTGTAACC | |
| mFBG | F:TTGGAATTCCCAGGGACTGCTCTCAAGCAATG |
| R:CGGGCAGCGGCCGCTGCCCGCTTACGCCTGCCT |