Figure 1.

Exponentially growing myometrial SMCs are more sensitive to the anti-proliferative effect of heparin than are cells released from quiescence. Exponentially growing SMCs were treated with 10% FCS in the presence or absence of NAC heparin (10 to 300 μg/ml) for 2 days. Quiescent cells were obtained by placing the cells in 0.25% FCS for 3 days. Cells were then released from growth arrest by the addition of 10% FCS in the presence or absence of NAC heparin (10 to 300 μg/ml) and were counted when confluent using a Coulter Counter. The dashed line represents the cells released from growth arrest and the solid line represents exponentially growing cells.