Figure 5.

Heparin inhibits the motility of myometrial and leiomyoma SMCs. Cells were plated to confluence and a wound was created using a P200 pipette tip. 10% FCS in the presence or absence of NAC heparin (10 to 500 μg/ml) was added and cells were fixed when the 10% FCS control wound was confluent. Hoechst 33258-containing medium was added to the fixed cells and fluorescent images were taken. The number of cells migrating into the wound was determined using image analysis software. Solid lines represent myometrial (Myo) and leiomyoma (Fib) SMCs from patient D, and dashed lines represent SMCs from patient B (Tables 1 and 2) ▶ . SEM are shown in Table 3 ▶ .