Figure 7.

Differential expression of EG-VEGF (green) and VEGF (red) in four individual PCOS specimens. A–D: Parallel tissue sections were hybridized with [³³P]-labeled probes, autoradiographic films were digitized, overlaid, and false-colored as described in Materials and Methods. H&E-stained sections (E–H) are also shown. Note the generally nonoverlapping expression pattern of each gene, with VEGF having a more restricted expression, most consistently associated with the lining of several individual cysts. EG-VEGF is expressed around a minority of VEGF-positive (A, B) and around all VEGF-negative cysts, as well as extensively in the stroma. Weak VEGF expression is noted in the fallopian tube mucosa (C, G), where EG-VEGF expression is absent. Scale bar, 5 mm.