. 2003 Jun;162(6):1913–1926. doi: 10.1016/S0002-9440(10)64325-8

Table 1.

Quantitative Computer-Assisted Analysis of Blood Vessel Size in Tet-FGF-2 and AS-VEGF/Tet-FGF-2 Xenografts

	Tetracycline	Number of measurements	Blood vessel diameter, μm		Large-sized vessels, area > 1000 μm² (% of total)
	Tetracycline	Number of measurements		Range	Large-sized vessels, area > 1000 μm² (% of total)	Mean ± SE
Tet-FGF-2 cells	−	766	5.1–172.8	23.5 ± 8.4	20.0 ± 3.0
	+	950	4.9–63.4	13.1 ± 4.2	3.0 ± 1.0
AS-VEGF/Tet-FGF-2 cells	−	622	4.8–47.8	11.6 ± 3.6	3.0 ± 1.5
	+	587	5.2–29.4	9.5 ± 3.2	0.8 ± 0.4

Tet-FGF-2 and AS-VEGF/Tet-FGF-2 tumors from mice treated with tetracycline or not (five tumors per group) were immunostained with anti-CD31 antibody. Then, quantitative computer-assisted image analysis was performed on digitized images of the indicated numbers of CD31⁺ tumor blood vessels. The percentage of large caliber vessels (vessel area > 1,000 μm²) was also calculated.