Figure 5.

VEGFR-3 expression is limited to lymphatic endothelium in normal and inflamed skin and absent on skin DC. To determine whether VEGFR-3 is also expressed on DC in the skin, we examined normal skin tissue sections that were double-stained for VEGFR-3 (red) and LYVE-1 (green) (A). VEGFR-3 was present and co-stained with LYVE-1 on lymphatic vessels (yellow), but was not expressed by leukocytes, including DC. Similar stainings with VEGFR-3 (red) and LYVE-1 (green) in inflamed skin (B) did not induce novel expression of VEGFR-3 on DC. The expression of VEGFR-3 and LYVE-1 was limited to lymphatic endothelium (yellow) that co-stained for both antibodies. Magnification, (A and B) ×200 (Epidermis is on the top).