VEGFR-3 staining is intracellular in uninflamed corneas, but membranous in inflamed corneas. To localize the cellular expression of VEGFR-3, we stained unfixed whole-mount corneas with VEGFR-3. Unfixed tissue failed to express VEGFR-3 (A). Similar staining of acetone-fixed corneal whole-mounts, however, showed granular cytoplasmic staining as shown with serial confocal sections of a single cell (B). Staining of unfixed inflamed corneas demonstrated VEGFR-3 expression on the cell surface (C). Magnification, (A–C) ×1000.