Figure 1.
MMPIs inhibit angiogenesis in vitro. 17-day-old fetal mouse metatarsals were treated with marimastat (open circle), CH3921 (filled circle), CH5902 (open triangle), or vehicle for 10 days. After 10 days, cultures were stained for PECAM-1. A: The total area of PECAM-1-positive capillary-like structures was determined by computerized image analysis. Data are expressed as treatment control of sextuple cultures ± SEM. The experiment was repeated three times. B: Representative images of control cultures, marimastat-, CH5902-, and CH3921 (10−6M)-treated cultures are shown after staining for PECAM-1. Original magnification, ×40.