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Oligonucleotide Sequences and Reaction Conditions for qRT-PCR
| Primer position | Probe position | Primer cc (nM) | Probe cc (nM) | Mg2+ cc (mM) | Sequence | |
|---|---|---|---|---|---|---|
| β-actin | 1020+ | 100 | 4 | ATCAAGATCATGCTCCTCCTGAGCGC | ||
| 997+ | 400 | CCCTGGCACCCAGCAC | ||||
| 1067− | 400 | GCCGATCCACACGGAGTAC | ||||
| TG | 564− | 100 | 4 | TCCAGGTACACAGCATCCGCTGGG | ||
| 537+ | 300 | CTTCATTTGCTCTTCAACGC | ||||
| 614− | 300 | ACATACTCCTGCCGCTCCTC |
