Figure 3.

SR-B1 and ABCA-1 in HK-2 cell extracts obtained 4 hours following the addition of an Fe challenge. Paralleling the changes observed in renal cortex, significant reductions in each protein were observed in the Fe-challenged cells, compared to the controls (Cont) (n = 6 separate cell preparations for each of the 4 groups). As with the renal cortical samples, ABCA-1 expression was, in general, remarkably lower than SR-B1, reflecting the fact that low levels of ABCA-1 exist in non-steroidogenic tissues.