Table 1.

Mouse and Human Primers Used for Assessment of ABCA-1, SR-B1, and LDL-R mRNAs in Mouse Renal Cortex or in HK-2 (Human) Proximal Tubular Cells

Genes	Primer sequences	PCR conditions	Product size
Mouse	5′-CAA TCG GAA AAC CAT TTT GG-3′	94°C, 60 s; 55°C, 60 s;	198 bp
LDL-R	5′-TGT GAC CTT GTG GAA CAG GA-3′	72°C, 60 s; 23 cycles
Mouse	5′-CTG CCA TTT GCA GTG GCA AAG TGG-3′	94°C, 60 s; 55°C, 60 s;	439 bp
GAPDH	5′-TTG TCA TGG ATG ACC TTG GCC AGG-3′	72°C, 60 s; 23 cycles
Human	5′-CAA TGT CTC ACC AAG CTC TG-3′	94°C, 60 s; 55°C, 60 s;	260 bp
LDL-R	5′-TCT GTC TCG AGG GGT AGC TG-3′	72°C, 60 s; 23 cycles
Human	5′-CTG TGG GTG AGA TCA TGT GG-3′	94°C, 60 s; 55°C, 60 s;	215 bp
SR-B1	5′-GCC AGA AGT CAA CCT TGC TC-3′	72°C, 60 s; 23 cycles
Human	5′-CAA CTA CAA AGC CCT CTT TG-3′	94°C, 60 s; 55°C, 60 s;	310 bp
ABCA-1	5′-CTT GGC TGT TCT CCA TGA AG-3′	72°C, 60 s; 30 cycles
Human	5′-GTC TTC ACC ACC ATG GAG AAG-3′	94°C, 60 s; 55°C, 60 s;	490 bp
GAPDH	5′-GCT TCA CCA CCT TCT TGA TGT CAT C-3′	72°C, 60 s; 23 cycles