Figure 2.

Oligomerization of wild-type and mutated COMP. COS7 cells were transiently transfected with wild-type COMP (lanes 1, 4, 7 and 10), mutated COMP (lanes 2, 5, 8 and 11), or vector alone (lanes 3, 6, 9 and 12) and cultured for 48 hours at 37°C under serum-free conditions. Cell lysates (1–3 and 7–9) and conditioned media (4–6 and 10–12) were analyzed by immunoblotting with the anti-COMP antibody to recognize the N-terminal fragment of COMP. The samples were treated with SDS sample buffer in the (1–6) absence or (7–12) presence of 2-mercaptoethanol (ME).