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. 2000 May 23;97(12):6630–6633. doi: 10.1073/pnas.110152897

Figure 1.

Figure 1

Spontaneous chromosomal fragmentation in NHEJ-deficient MEFs. Arrows indicate the aberration. (a–c) Images of 4′,6-diamidino-2-phenylindole (DAPI)-stained metaphase chromosomes. (d) Both DAPI staining and SKY analysis. (a) Lig4−/−; broken chromatid. (b) DNA-PKcs−/−; two marker chromosomes (acentric fragments). (c) Ku70−/−; single chromatid gap. (d) Ku70−/−; double chromatid break. (Left) DAPI stain; (Right) SKY analysis. The fragment and the adjacent chromosome show identical staining by SKY, suggesting that the fragment arose from the termini of the adjacent chromosome 7.