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. 2003 Aug;163(2):753–762. doi: 10.1016/S0002-9440(10)63702-9

Figure 1.

Figure 1.

Effects of ET-1 on the synthesis, secretion and activation of MMP-2 in KS IMM cells. A: ET-1 stimulates MMP-2 secretion from KS IMM cells. MMP-2 secretion was measured in conditioned media from cells treated with different concentrations of ET-1 for 24 hours using ELISA kit. Data are presented as means of results from three experiments each performed in duplicate. Bars ± SD. a, P ≤ 0.0001; b, P ≤ 0.005. B: Left, gelatinolytic activity of MMP-2 was studied in conditioned media from cells by SDS-PAGE gelatin zymography. Cells, after starvation, were grown in serum-free medium for 24 hours in the absence (C; control) or presence of 100 nmol/L ET-1. Right, expression of 518 mRNA transcripts for MMP-2 was detected by RT-PCR analysis. Primers for the amplification of GAPDH gene were used as controls. Data shown are PCR products of KS IMM cells grown in serum-free medium for 8 hours in the absence (C; control) and in presence of 100 nmol/L of ET-1. PCR products for MMP-2 and GAPDH were shown as visualized by ethidium bromide.