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. 2003 Aug;163(2):753–762. doi: 10.1016/S0002-9440(10)63702-9

Figure 2.

Figure 2.

Effect of ET-1 on the synthesis, secretion, and activation of MT1-MMP in KS IMM cells. A: Total RNA (20 μg) extracted from untreated cells (C; control) or treated for 8 hours with 100 nmol/L ET-1 was analyzed by Northern blotting using MT1-MMP cDNA probe. The filter was also hybridized with a GAPDH probe, as a control for RNA loading. B: Fold-increase of MT1-MMP mRNA over control by densitometric analysis of autoradiographic bands normalized to those of GAPDH. Bars ± SD; *, P ≤ 0.001 compared to control. C: Extracts of untreated KS IMM cells (C; control) or treated with 100 nmol/L ET-1 or ET-3 for 24 hours was tested for MT1-MMP (the 65-kd latent form and the 63-kd active form) by Western blotting.