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Specificity of ATM monoclonal antibody 11G12. Western blotting (A) shows expression of a 370-kd band in 3 LCLs from normal donors and absence of the same band in the LCLs from three A-T donors with truncating ATM mutations of both alleles. Immunohistochemistry using the 11G12 antibody produced strong nuclear staining in cytospin preparations of the normal donor LCLs, whereas the LCLs from the A-T donors were negative (B).
