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. 2003 Aug;163(2):423–432. doi: 10.1016/S0002-9440(10)63672-3

Figure 2.

Figure 2.

ATM expression in bone marrow. ATM-positive cells of the bone marrow were small and comprised 5% to 10% of the total cellular compartment. Dual-labeling was performed for ATM (brown) and a range of hematopoietic differentiation markers (dark gray/black). a: Dual-labeling with an antibody specific for CD34; the majority of CD34-expressing cells were ATM-negative. ATM expression was apparent in more mature lymphoid cells, including those that expressed CD45 and CD20 (b and c, respectively). However, a proportion of ATM-positive cells were not labeled with either CD20 or CD45. Triple-labeling with ATM, CD20, and either CD138, CD68, or CD3 revealed that these cells were mostly either plasma cells, macrophages, or T-lymphocytes. Inset of c shows triple-labeling for ATM (brown), CD20 (purple), and CD138 (gray/black). Cells of the erythroid series (shown in d by staining with antibodies to glycophorin A) and those of the myeloid series (e, by staining with antibodies to myeloperoxidase) were consistently ATM-negative. Megakaryocytes (f, stained for CD42b) showed weak to moderate staining with the ATM antibody.