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. 2003 Aug;163(2):543–552. doi: 10.1016/S0002-9440(10)63682-6

Figure 2.

Figure 2.

BI-1 expression in prostate carcinoma (PCa) and stromal tissue. A: Before quantitative RT-PCR the amount and integrity of total RNAs derived from paired laser-captured microdissected (LCM) samples were analyzed on an Agilent Pico LabChip. The bands for 28s rRNA and 18s rRNA are indicated (M, Ambion RNA 6000 ladder; P, normal prostate; PCa, prostate carcinoma). B: BI-1 expression and clinicopathological features of the patients analyzed in the present study. Prescreened total RNAs isolated from LCM paired normal prostates and prostate tumors from seventeen radical prostatectomies were analyzed by two-step real-time RT-PCR for BI-1 expression relative to β-actin. Factors of up-regulation of BI-1 expression in tumor specimens as compared to matched normal prostate tissues are shown. (* indicates factor of up-regulation of BI-1 expression in comparison to tumor-free tissue normalized against β-actin). C: BI-1 expression in stromal tissues. Total RNAs derived from LCM paired stromal tissue and epithelial tissue (tumor-free) from five radical prostatectomies were analyzed as described in B (** indicates factor of down-regulation of BI-1 expression in stromal tissue in comparison to tumor-free tissue normalized against β-actin).