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. 2003 Aug;163(2):543–552. doi: 10.1016/S0002-9440(10)63682-6

Figure 3.

Figure 3.

Non-radioactive in situ hybridization of BI-1 mRNA in prostate tissue. A: BI-1mRNA expression is mainly restricted to prostate carcinoma cells, whereas only weak BI-1 expression is observed in the stromal compartment using a BI-1-specific antisense cRNA probe for hybridization (scale bar, 200 μm). B: In situ hybridization using a BI-1-specific sense cRNA probe (negative control) confirms the specificity of the hybridization signals. C and D: In one case an inflammatory infiltrate (arrow) was observed in the vicinity of carcinoma cells (arrowheads) within the prostate stroma after hematoxylin-eosin staining. In situ hybridization with a BI-1 antisense cRNA probe revealed strong expression of BI-1 in infiltrating cells (inset, higher magnification of BI-1 hybridization signals on infiltrating cells).