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. 2003 Sep;163(3):1157–1166. doi: 10.1016/S0002-9440(10)63475-X

Figure 3.

Figure 3.

Both cAMP and cGMP are elevated in BBECs treated with EtOH. Confluent monolayers of BECs were stimulated with 100 mmol/L of EtOH and flash-frozen in cell lysis buffer (see Materials and Methods). Cell homogenates were boiled and assayed for cAMP and cGMP concentrations. EtOH stimulated a significant increase in cAMP concentration at ∼30 minutes of treatment. EtOH also stimulated a measurable increase in cGMP with the maximal cGMP concentration observed at 45 minutes. Cyclic nucleotide levels were expressed as pmol cyclic nucleotide per mg total cellular protein. Bars represent SEM of separate experiments performed in triplicate (n = 6). Significance (P < 0.05) indicated by an asterisk.

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