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. 2003 Sep;163(3):1157–1166. doi: 10.1016/S0002-9440(10)63475-X

Figure 4.

Figure 4.

Substimulatory concentrations of cAMP and cGMP combine to stimulate CBF increases in BBECs. Confluent monolayers of ciliated BECs were stimulated with 1 nmol/L of 8Br-cAMP, 1 nmol/L of 8Br-cGMP, or both for 1 to 4 hours and CBF measured. At low concentrations (1 nmol/L), neither 8Br-cAMP nor 8Br-cGMP stimulated a significant change in CBF as compared to media controls (A). However, the combination of both 8Br-cAMP and 8Br-cGMP at 1 nmol/L stimulated an ∼2 to 3 Hz increase in CBF during the same incubation period (A and B). Preincubation of the cells with either 1 μmol/L of KT5720 or 100 nmol/L of 8-CPT-cGMPS blocked the CBF stimulatory effect of combined low-level cyclic nucleotides (B). CBF was expressed as cycles per second (Hz). Bars represent SEM of separate experiments performed in triplicate (n = 9). Significance (P ≤ 0.05) indicated by an asterisk.

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