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. 2003 Sep;163(3):1001–1011. doi: 10.1016/s0002-9440(10)63460-8

Figure 4.

Figure 4.

Effect of IGF-IR function on constitutive and inducible HIF-1α expression. A: Parental L3.6pl cells were serum-deprived in 1% FBS-MEM overnight, treated for the indicated times with rhIGF-I (100 ng/ml), and subjected to Western blot analysis of HIF-1α protein in nuclear extracts. B: Western blot analysis of constitutive HIF-1α nuclear translocation in pcDNA- and IGF-IR DN-transfected L3.6pl cells. Cells were incubated for 48 hours in 10% FBS-MEM to evaluate the possible effects of an IGF-I autocrine loop on HIF-1α activation. Nuclear protein extracts from each cell line were analyzed by Western blot analysis. By densitometry, IGF-IR DN-transfected cells showed approximately half the nuclear translocation of HIF-1α as that of control (pcDNA-transfected) cells.