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RT-PCR analysis of ALK cDNA. RT-PCR was performed with primers designed to amplify respectively the 3′-end (lanes 1 and 2) or the 5′-end (lanes 3 and 4) of ALK. Lanes 1 and 3 show the absence of any product using cDNA of the tumor. Lanes 2 and 4: PCR was performed with cDNA from an ATIC-ALK positive control case. Lane 5 is a positive control for the cDNA synthesis showing amplification of the CIZ gene, starting from cDNA of the tumor.
