Figure 1.

Schematic representation of the human Ikaros gene and its isoforms. DNA binding, repression/activation domains, and dimerization domains are indicated. The narrow open rectangles represent the specific zinc finger domains. Note that alternative splicing yields mRNA isoforms that encode truncated proteins that differ in the number of N-terminal zinc fingers. The dimerization domain is shared by all eight isoforms. Solid black arrows indicate sites corresponding to nested PCR primers as detailed in Materials and Methods (S1 sense primer, reaction 1; AS1 anti-sense primer, reaction 1; S2 sense primer, reaction 2; AS2 anti-sense primer, reaction 2).