Table 3.
Summary of the Data Obtained by Real-Time RT-PCR Analysis of 12 Selected Genes in an Independent Series of 43 Astrocytic Gliomas
| Gene | Means in each diagnostic group* | P values for selected comparisons using one-way analysis of variance models on log-transformed data† | ||||||
|---|---|---|---|---|---|---|---|---|
| AII (n = 9) | AAIII (n = 10) | pGBIV (n = 17) | sGBIV (n = 7) | AII vs. AAIII | AII vs. GBIV | AAIII vs. GBIV | pGBIV vs. sGBIV | |
| FOXMI | 1.65 | 5.38 | 3.99 | 5.79 | 0.0118 | 0.0317 | >.2 | >.2 |
| VEGFA | 1.21 | 2.34 | 9.33 | 5.77 | >.2 | 0.0004 | 0.0023 | 0.0590 |
| TOP2A | 7.82 | 22.47 | 22.36 | 193.53 | 0.0233 | 0.0000 | 0.0140 | 0.0001 |
| COL4A2 | 16.38 | 27.78 | 46.53 | 33.91 | 0.0402 | 0.0030 | >.2 | >.2 |
| IGFBP4 | 4.91 | 8.93 | 16.58 | 10.00 | >.2 | 0.0073 | 0.0154 | >.2 |
| CENPF | 10.05 | 29.34 | 31.04 | 127.84 | 0.0332 | 0.0000 | 0.0095 | 0.0040 |
| MGP | 2.43 | 6.66 | 11.19 | 9.91 | 0.0034 | 0.0004 | >.2 | >.2 |
| SELL | 15.07 | 5.36 | 2.81 | 6.09 | >.2 | >.2 | >.2 | >.2 |
| RYRI | 18.12 | 3.03 | 2.23 | 3.51 | >.1 | >.1 | >.2 | >.1 |
| ADD3 | 1.52 | 0.84 | 0.95 | 0.37 | 0.0272 | 0.0113 | >.2 | >.2 |
| CAMK2G | 1.03 | 0.57 | 0.47 | 0.58 | >.1 | 0.0170 | >.2 | >.2 |
| ABLIM | 3.60 | 3.18 | 2.28 | 3.79 | >.2 | >.1 | >.2 | 0.0445 |
*The mean values calculated for the mRNA expression in each group of tumors (astrocytoma WHO grade II, AII; anaplastic astrocytoma WHO grade III, AAIII; primary glioblastoma WHO grade IV, pGBIV; secondary glioblastoma WHO grade IV, sGBIV) are given.
†P values for testing differences between the group means for four comparisons of interest, i.e, AII vs. AAIII, AII vs. all glioblastomas, AAIII vs. all glioblastomas (GBIV), pGBIV vs. sGBIV.