Figure 4.

Effects of various metabolic inhibitors on the nocodazole-induced apoptosis in mitotic spindle checkpoint-proficient NCI-H460 cells. PD98059, LY294002, wortmannin, SB203580, and caspase inhibitors were added simultaneously with 200 nmol/L of nocodazole treatment, while thymidine and staurosporine were added 12 hours before and after the initiation of nocodazole treatment, respectively. Note that staurosporine treatment markedly reduces apoptotic cells 48 hours after the initiation of nocodazole treatment.