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. 2003 Sep;163(3):1167–1175. doi: 10.1016/S0002-9440(10)63476-1

Figure 1.

Figure 1.

μ-Opioid receptor expression in Huh.8, FCA-1, and Huh7 cells. RT-PCR analysis of μ-opioid receptor gene expression. Total RNA was extracted from Huh.8, FCA-1, and Huh7 cells and subjected to RT-PCR using specific primers for μ-opioid receptor. Sizes are estimated from DNA ladder (100-bp fragments) co-electrophoresed as markers. Lane 1: Markers; lane 2: Huh.8 cells; lane 3: FCA-1 cells; lane 4: Huh7 cells; lane 5: NT2-N cells as a positive control (+); lane 6: negative control (-, the same cells as used in the positive control but processed without reverse transcriptase).